Isolation of a new toxic protease from a strain of Aeromonas salmonicida subspecies achromogenes
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چکیده
A new toxic protease was isolated from Aeromonas salrnonicida subspecies achromogenes extracellular products (ECP). It was detected in 5 of 9 strains of the organism tested. The protease was purified by anion-exchange fast protein liquid chromatography (FPLC) on Mono Q HR 5/5, and FPLC gel filtration on Superose 12 HR 10/30. The LDso of the purified protease for juvenile Atlantic salmon Salrno salar L. was 0.03 pg protein g' fish. The molecular weight of the protease was estimated to be 20 kD by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and it possessed 2 isoelectric points (PIS) of 6.2 and 6.4. The protease was completely inhbited by EDTA Indicating it to be a metallo-protease.
منابع مشابه
Draft Genome Sequence of Aeromonas salmonicida subsp. achromogenes AS03, an Atypical Strain Isolated from Crucian Carp (Carassius carassius) in the Republic of Korea
We present the draft genome sequence of Aeromonas salmonicida subsp. achromogenes strain AS03, an atypical A. salmonicida strain that causes erythrodermatitis in crucian carp (Carassius carassius). This is the first genome sequence report of A. salmonicida subsp. achromogenes, one of the four subspecies of atypical A. salmonicida.
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